Master GREEN 200 Manual de usuario descargar pdf (Pagina 8)

1-2 Introduction

Purpose of the Kit

About the Kit

The SYBR Green PCR Master Mix is a convenient premix of all the

components, except primers, template and water necessary to perform

real-time PCR using SYBR

Green I Dye. Direct detection of

polymerase chain reaction (PCR) product is monitored by measuring

the increase in fluorescence caused by the binding of SYBR Green dye

to double-stranded (ds) DNA.

One-Step or Two-Step RT-PCR can be performed using the SYBR

Green RT-PCR Reagents Kit (refer to “Materials and Equipment” on

page 1-3).

In RNA quantitation assays, the SYBR Green PCR Master Mix is used

in the second step of a two-step reverse-transcription polymerase chain

reaction (RT-PCR) protocol. In a One-Step RT-PCR protocol,

MultiScribe™ Reverse Transcriptase and RNase Inhibitor are added to

the SYBR Green PCR Master Mix.

The SYBR Green PCR Master Mix is designed for use with the

ABI P

RISM

7700 Sequence Detection System (SDS), the ABI PRISM

7900HT SDS, the ABI PRISM

7000 SDS,

or the GeneAmp

5700 SDS.

For the best quantitation results, use the following:

Primer Express

™

software for primer design

Applied Biosystems reagents

Applied Biosystems universal thermal cycling conditions

About This

Protocol

This protocol describes how to perform PCR and One-Step or Two-Step

RT-PCR using SYBR Green PCR Master Mix.

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Master GREEN 200 Manual de usuario Pagina 8