3-6 Reverse Transcription
Guidelines
Follow the guidelines below to ensure optimal RT performance.
Poly A
+
RNA samples are not recommended for 18S experiments
because most rRNA has been removed from them.
A 100-µL RT reaction will efficiently convert a maximum of 2 µg
total RNA to cDNA. Perform multiple RT reactions in multiple wells
if using more than 2
µg total RNA.
Use only random hexamers to reverse transcribe the total RNA
samples for gene expression assays.
Preparing the
Reactions
The following procedure describes the preparation of four different test
samples for RT. Scale the recommended volumes accordingly for the
number of samples needed using the TaqMan Reverse Transcription
Reagents
(N808-0234).
Note The kit contains sufficient quantities to perform 200 RT reactions with a
reaction size of 10 µL.
Comentarios a estos manuales