Data Analysis 5-3
Interpreting the Results
Passive Reference
ROX
The Passive Reference (ROX) is a dye molecule included in the SYBR
Green PCR Master Mix that does not participate in the PCR
amplification. On the ABI P
RISM
®
7700 SDS, the ABI PRISM
®
7900HT
SDS, the ABI P
RISM
®
7000 SDS, and the GeneAmp
®
SDS, the Passive
Reference provides an internal reference to which the SYBR
Green/dsDNA complex signal can be normalized during data analysis.
Normalization is necessary to correct for well-to-well fluorescent
fluctuations.
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